Collectively, they represent the stateoftheart for colorimetric detection and quantitation of total protein. Biovision biotin quantitation kit colorimetric, k811100. Fluoreporter biotin quantitation assay kit for biotinylated proteins f30751 figure 1. Meridian road rockford, ll 61105 usa 815 9687316 fax number description. It consists of labeling cell surface proteins with a biotin reagent before lysing the cells, and isolating these tagged proteins by. Table 1 comparison of common dna, rna and protein quantitation assays method detection. Conjugation of oligosaccharides to probes and surfaces is useful in the development of biochemical assays to assess carbohydrateprotein interactions. Pdf quantitative recovery of biotinylated proteins from. Biotin quantitation kit colorimetric k811 biovision. Enzymatic method for determining lamino acids lamino. In parallel, a set of known biotin concentrations was used to construct a standard. Application methods p a g e 1 080409 nucleic acid and protein quantitation methods prepared by. Direct streptavidinbinding assay does not accurately quantitate.
Assessment of biotin interference with qualitative pointof. The bca protein assay kit provides a simple, rapid, detergent tolerant up to 5% procedure for determining the concentration of proteins in solution. Pdf biotin switch assays for quantitation of reversible. Pierce biotin quantitation kit thermo fisher scientific. Pierce offers seven colorimetric assays for detection and quantitation of total protein. The fluorescence biotin quantitation kit is requires only 10 l of sample. The thermo scientific pierce biotin quantitation kit contains a premix of haba and avidin and a biotinylated horseradish peroxidase hrp positive control. Thermo scientific pierce biotin quantitation kit includes aliquots of haba reagent. Pdf ingel detection of biotinprotein conjugates with a. The assay is performed in a 96well microtiter plate format and measured within 15 minutes. Proceed to the calculation of moles of biotin per mole of protein section. The protein quantification kit provides a simple and rapid procedure for determining the concentration of protein in solution.
Biotin quantitation with quanttag kit from vector laboratories. This kit can be used to determine accurately the labeling efficiency of biotinlabeled molecules. The biotin 3 end dna labeling kit from pierce biotechnology incorporates biotinylated ribonucleotides onto the 3 end of dna strands by using terminal deoxynucleotidyl transferase tdt. Thermo scientific pierce biotin quantitation kit available on gsava contract for federal government customers only. Thermo scientific pierce biotin quantitation kit ez. The activated proenzyme then catalyzes the splitting of p. This labeling strategy has the advantage of localizing the biotin to the 3 end of the probe where it is less likely to interfere with hybridization or sequencespecific binding of proteins. For proteins of lower molecular weight or multiple biotin labels, less protein can be used. Ez biotin quantitation kit from pierce biocompare product. The quanttag biotin kit bdk2000 is designed to determine the amount of free biotin in solution or the number of biotins attached to nucleic acids, proteins, or other macromolecules.
The assay employs haba 4hydroxyazobenzene2carboxylic acid, a reagent that shows dramatic. The pierce mass spec sample prep kit for cultured cells contains all the necessary reagents and enzymes to prepare up to 20 samples 1 million cells. L of the habaavidin premix solution from step 2 to the cuvette. Haba 24hydroxyazobenzene benzoic acid and avidin are the basis of the kit. The accublue high sensitivity assay is also available without any dna standards 31008, for those that wish to use their own standard.
A modified microbradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents and. The ez biotin quantitation kit from pierce biotechnology is used to measure the biotin levels in protein samples and thereby measure the molar ratio of biotin to protein in the sample. This kit can be used to determine accurately the labeling efficiency of biotin labeled molecules. They are all wellcharacterized, robust assays that provide consistent, reliable results. Ingel detection of biotinprotein conjugates with a green fluorescent streptavidin probe. The endotoxin concentration in a sample is measured using the pierce lal chromogenic endotoxin quantitation kit via a chromogenic signal generated in the presence of endotoxins. The tdt prefers to label singlestranded dna, but can also label doublestranded dna with blunt or 3 overhangs, but at a lower efficiency. The assay employs haba 4hydroxyazobenzene2carboxylic acid, a reagent that shows dramatic spectral changes when bound to avidin. This colorimetric biotin quantitation kit provides a convenient method for estimating the molar ratio of biotin to protein in biotinprotein conjugates or for quantitating biotin concentration in a solution. Determine the molar ratio of biotin incorporated into a protein using the habaavidin method. Ample materials are provided to perform 100 assays in cuvette format or 500 assays in a 96well format. Biotin vitamin h is an important biomolecule that has been widely used in biotinylation reactions to label antibodies or other proteins of interest. Thermo scientific pierce immunostain enhancer 14 thermo scientific dapi stain 15 thermo scientific hoechst 33342 stain 1617 thermo scientific dylight conjugates 1819 licor odyssey instrument products 2021 fluorescence biotin quantitation kit 2223 fluorescent protein gel stains 24.
Piercetm bca protein assay kit thermopierce, catalog number. Colorimetric biotin assay kit mak171 technical bulletin. Our kit is more accurate than the other suppliers fluorescent biotin assay brand i. Fluorescence biotin quantitation kit thermo fisher scientific. Ezlink sulfonhs biotin sulfosuccinimidyl6 biotin amidohexanoate, 25mg. The thermo scientific ez biotin quantitation kit contains premeasured doses of the avidinhaba mixture and biotinylated horseradish peroxidase positive control to simplify reagent preparation and minimize the amount of waste generated. The thermo scientific biotin 3 end dna labeling kit uses tdt to incorporate biotinylated ribonucleotides onto the 3 end of dna strands. The habaavidin premix is supplied in convenient noweigh microtube packaging, which eliminates the difficulties as sociated with weighing small quantities of reagent. Many of the biotin labeling kits from pierce use an nhsbiotin reagent without the chromogenic fluorophore. Quicktiter aav quantitation kit provides an efficient system for rapid quantitation of aav titer for both viral supernatant and purified virus. Dilutions must be accounted in the calculation step. Urine solutions for the biotin doseresponse experiment were prepared by pooling two nonpregnant patient urine samples, resulting in a urine matrix with an hcg concentration pdf. Cell biolabs high sensitivity protein quantitation assay kit utilizes a fluorogenic detection dye to measure primary aliphatic amines peptides or proteins in various samples with much greater sensitivity than the conventional colorimetric protein determination methods. Cell surface protein biotinylation and analysis bioprotocol.
Biotin quantitation kit colorimetric k811 biovision, inc. The thermo scientific fluorescence biotin quantitation kit requires only 10. Using a chromogenic biotin reagent offers many advantagesyou can use simple uv quantitation to determine biotin incorporation with no need for the haba assay, an additional assay required to determine the extent of biotin incorporation. Pierce biotin quantitation kit thermo fisher scientific the thermo scientific pierce biotin quantitation kit contains a premix of. All samples and standards should be run in duplicate. Protein quantitation kit is used for determining the concentration of protein in solution. Biotin quantitation kit, quanttag vector laboratories, inc. The thermo scientific pierce lal chromogenic endotoxin quantitation kit measures the amount of endotoxin in a protein, peptide or antibody sample using the limulus amebocyte lysate lal assay. Samples can be measured on a microplate absorbance reader at 405nm. Thermo scientific pierce lal chromogenic endotoxin. Bacterial endotoxin catalyzes theactivation of a proenzyme in the modified limulus amebocyte lysate lal. Pierce biotechnology po box 117 815 9680747 3747 n. Biotin, a 244 dalton vitamin found in tiny amounts in all living cells, binds with high affinity to avidin, streptavidin and thermo scientific neutravidin biotinbinding protein. Amplite colorimetric biotin quantitation kit aat bioquest.
Highly purified distilled and deionized water was used throughout. Thermo scientific pierce biotin quantitation kit includes aliquots of haba reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins. Development of a sensitive, rapid, biotinstreptavidin. Thermo scientific pierce protein assay technical handbook. Thermo scientific pierce fluorescence biotin quantitation kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled. Assay is suitable for measuring protein concentration in the range of 0. The thermo scientific pierce lal chromogenic endotoxin quantitation kit is an efficient, quantitative endpoint assay for the detection of gramnegative bacterial endotoxins. The thermo scientific pierce biotin quantitation kit contains a premix of. Biotin quantitation reagents and kits fisher scientific. Biotin 3 end dna labeling kit from pierce biotechnology. It isnt always easy to find a trusted source of lab equipment and chemicals like this this pierce lal chromogenic endotoxin quantitation kit. Thermo scientific pierce biotin quantitation kit ez biotin.
Biotin quantitation reagents and kits thermo scientific pierce biotin quantitation kit haba dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins. Pierce bca protein assay kit brigham young university. The pierce biotin quantitation kit contains a premix of haba and avidin and a biotinylated horseradish peroxidase hrp positive control. The assay utilizes haba 24hydroxyphenylazobenzoic acid, a reagent that shows dramatic spectral changes when bound to avidin. May 24, 2007 the biotin 3 end dna labeling kit from pierce biotechnology incorporates biotinylated ribonucleotides onto the 3 end of dna strands by using terminal deoxynucleotidyl transferase tdt. Both nucleic acid and protein quantitation can be performed in single tube or microplate assays. What is the difference between the trilink biotin labeling. Piercea protein assay kit bc thermo fisher scientific. The quanttag biotin kit bdk2000 from vector laboratories is designed to determine the amount of free biotin in solution or the number of.
This colorimetric biotin quantitation kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin protein conjugates or for quantitating biotin concentration in a solution. The habaavidin premix is supplied in convenient thermo scientific noweigh microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent. Sulfosuccinimidyl6biotinamido hexanoate nhslcbiotin was purchased from pierce chemical rockford, il. The thermo scientific pierce mass spec sample prep kit for cultured cells is an easytouse, comprehensive kit for preparation of clean peptide mixtures from cultured cells for ms analysis. The accublue high sensitivity dsdna quantitation kit offers sensitive and selective detection of purified dsdna samples with a linear detection range from 0. Ez biotin quantitation kit was purchased from pierce usa. Sensolyte biotin quantitation kit fluorimetric size. The kit has a detection sensitivity limit of 1 x 109 gcml genome copyml for unpurified aav2 or aavdj supernatants, or 5 x 10 10 gcml for purified aav sample from any serotype, which is sufficient for mid or hightiter aav samples. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. The convenient assay can be performed either in a cuvette or in a microplate. The haba biotin quantitation kit provides a convenient method for estimating the molar ratio of biotin to protein in biotinprotein conjugates or for quantitating biotin concentration in a solution. An assay for biotin that uses europium coupled to streptavidin and. Urine specimens were stored at 28 c, protected from light, for no longer than 7 days prior to the measurement of biotin and creatinine concentration roche crej2, c701.
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